Substrate porosity and morphological performance identify an effective growing medium for habanero pepper (Capsicum chinense Jacq. cv. Francisca) seedlings

Authors

  • Pandi Ariansah Agronomy and Horticulture Study Program, Undergraduate School of IPB University, Bogor, West Java 16680, Indonesia
  • Muh Agust Nur Fathoni Asian Agriculture Course, Graduate School of Agriculture, Ibaraki University, Inashiki, Ibaraki 300-0393, Japan
  • Krisantini Department Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, West Java 16680, Indonesia
  • Edi Santosa Department Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, West Java 16680, Indonesia

DOI:

https://doi.org/10.61511/jassu.v4i1.2026.3893

Keywords:

habanero pepper, nursery substrate, porosity, seedling quality

Abstract

Background: Habanero Francisca pepper (Capsicum chinense Jacq.) is an ornamental chili cultivar whose seedling quality is strongly influenced by the composition of the growing medium. Rice husk charcoal, cocopeat, and bamboo compost are commonly used nursery substrates because they improve water retention, aeration, and nutrient availability; however, information regarding their combined use for Habanero Francisca pepper seedlings remains limited. This study aimed to identify an effective growing medium composition based on substrate physical properties and seedling morphological responses. Methods: A randomized complete block design with one factor (growing medium composition) and four treatments was applied: M0 (commercial growing medium, control), M1 (rice husk charcoal: cocopeat: bamboo compost = 2:1:1), M2 (1:2:1), and M3 (1:1:2). Substrate properties, including bulk density, particle density, and porosity, were measured. Seedling growth was evaluated through plant height, leaf number, root length, root number, total fresh weight, and total dry weight. Data were analyzed using the F-test followed by Duncan’s Multiple Range Test at the 5% significance level. Findings: The composition of the growing medium significantly affected plant height, root length, root number, total fresh weight, and total dry weight, but had no significant effect on leaf number. The M2 treatment produced the highest porosity (82.11%) and superior seedling performance, with plant height of 9.55 cm, root length of 10.36 cm, total fresh weight of 0.55 g, and total dry weight of 0.07 g, compared with 5.69 cm, 4.05 cm, 0.25 g, and 0.01 g, respectively, in the control treatment. Higher porosity in M2 likely enhanced root development and resource uptake, resulting in greater biomass accumulation. Conclusion: A cocopeat-dominant medium (1:2:1) is the most effective composition for producing high-quality Habanero Francisca pepper seedlings, supporting the use of agricultural residues as a renewable, resource-efficient alternative to commercial peat-based media in pepper nurseries. Novelty/Originality of this article: This study is the first to integrate substrate porosity assessment with morphological performance evaluation to determine an effective nursery medium specifically for Habanero Francisca pepper, a cultivar for which such information has not previously been reported.

References

Al-Ajlouni, M. G., Othman, Y. A., Abu-Shanab, N. S., & Alzyoud, L. F. (2024). Evaluating the performance of cocopeat and volcanic tuff in soilless cultivation of roses. Plants, 13(16), 2293. https://doi.org/10.3390/plants13162293

Atzori, G., Pane, C., Zaccardelli, M., Cacini, S., & Massa, D. (2021). The role of peat-free organic substrates in the sustainable management of soilless cultivations. Agronomy, 11(6), 1236. https://doi.org/10.3390/agronomy11061236

Balliu, A., Zheng, Y., Sallaku, G., Fernández, J. A., Gruda, N. S., & Tuzel, Y. (2021). Environmental and cultivation factors affect the morphology, architecture and performance of root systems in soilless grown plants. Horticulturae, 7(8),243. https://doi.org/10.3390/horticulturae7080243

Caron, J., & Michel, J.-C. (2021). Understanding and optimizing the physical properties of growing media for soilless cultivation. In M. Asaduzzaman (Ed.), Advances in horticultural soilless culture (pp. 107–137). Burleigh Dodds Science Publishing. https://doi.org/10.19103/AS.2020.0073.06

Choudhary, V., & Machavaram, R. (2023). A comprehensive review of sustainable soil organic growing media for mat-type paddy seedling nurseries under Indian agronomical conditions. Journal of Soil Science and Plant Nutrition, 23(2), 1515–1534. https://doi.org/10.1007/s42729-023-01153-2

Durand, S., Fonteno, W. C., & Michel, J.-C. (2024). A review and analysis of particle size parameters and their relationships to physical properties of growing media. Soil Science Society of America Journal, 88(3), 652–666. https://doi.org/10.1002/saj2.20661

El Amrani, B. (2023). Exploring the importance of root architecture plasticity in plant adaptation to environmental constraints. Plant Species Biology, 38(5), 234–244. https://doi.org/10.1111/1442-1984.12410

Fathi, A. (2022). Role of nitrogen (N) in plant growth, photosynthesis pigments, and N use efficiency. Agrisost, 28, 1–8.

Gallegos-Cedillo, V. M., Nájera, C., Signore, A., Ochoa, J., Gallegos, J., Egea-Gilabert, C., & Fernández, J. A. (2024). Analysis of global research on vegetable seedlings and transplants and their impacts on product quality. Journal of the Science of Food and Agriculture, 104(9), 4950–4965. https://doi.org/10.1002/jsfa.13309

Goldan, E., Nedeff, V., Barsan, N., Culea, M., Panainte-Lehadus, M., Mosnegutu, E., & Irimia, O. (2023). Assessment of manure compost used as soil amendment: A review. Processes, 11(4), 1167. https://doi.org/10.3390/pr11041167

Homchat, K., & Ramphueiphad, S. (2022). The continuous carbonisation of rice husk on the gasifier for high yield charcoal production. Results in Engineering, 15, 100495. https://doi.org/10.1016/j.rineng.2022.100495

Irfan, M., Alotibib, M. M., Alotaibi, N. M., Ghoneim, A. M., Rehman, H., & Moghanm, F. S. (2024). Seed nutripriming with magnesium to improve seedling growth and yield in wheat: Consequences for seed nutrient reserves metabolism and photochemical efficiency. South African Journal of Botany, 165, 275–281. https://doi.org/10.1016/j.sajb.2023.12.016

Jayaprakash, K., & Balamurugan, V. (2022). Enhancement of plant growth and water-holding capacity by using Moringa gum and coco peat-based substrates with Arka microbial consortium. Research Journal of Agricultural Sciences, 13, 1709–1713.

Júnior, L. A. Z., de Andrade, E. A., Pereira, N., Tokura, L. K., Secco, D., & Carvalho-Zanão, M. P. (2022). Contribution of carbonized rice husk added to substrates in vegetable seedling production: Bioscience Journal, 38, e38029.

Khatri, R., Mandal, D. K., Adhikari, N., Basnet, P., Mishra, S., & Neupane, S. (2025). Evaluating the impact of different growing media on germination parameters and seedling growth of tomato (Solanum lycopersicum L.) in Bhojpur, Nepal. International Journal of Horticulture, 15.

Li, G., Cheng, H., Qiao, C., Feng, J., Yan, P., Yang, R., & Zhang, Z. (2025). Root-zone oxygen supply mitigates waterlogging stress in tomato by enhancing root growth, photosynthetic performance, and antioxidant capacity. Plant Physiology and Biochemistry, 222, 109744. https://doi.org/10.1016/j.plaphy.2025.109744

Maqbool, S., Hassan, M. A., Xia, X., York, L. M., Rasheed, A., & He, Z. (2022). Root system architecture in cereals: Progress, challenges, and perspectives. The Plant Journal, 110(1), 23–42. https://doi.org/10.1111/tpj.15669

Muñoz-Ramírez, L. S., Peña-Yam, L. P., Álvarez-Gil, M. A., Iglesias-Andreu, L. G., Avilés-Viñas, S. A., Canto-Flick, A., & Santana-Buzzy, N. (2020). Selection of habanero pepper F1 hybrids (Capsicum chinense Jacq.) in the Yucatán Peninsula, Mexico with high potential for different markets. Agriculture, 10(10), 478. https://doi.org/10.3390/agriculture10100478

Negi, M., Sharma, S., & Singh, M. (2025). Plant-driven soil dynamics: Exploring the role of roots, fungi, and soil traffic in shaping soil structure. Communications in Soil Science and Plant Analysis, 56(18), 2727–2746. https://doi.org/10.1080/00103624.2025.2524075

Nong, J., Zheng, Y., Lin, H., Zhu, X., Yang, R., Huang, L., Wei, Y., & Xu, Z. (2025). Optimal fertilizer formulation for maximizing bamboo shoot production while improving soil fertility. BMC Plant Biology, 25(1), 107. https://doi.org/10.1186/s12870-025-07307-x

Ogorek, L. L. P., Gao, Y., Farrar, E., & Pandey, B. K. (2025). Soil compaction sensing mechanisms and root responses. Trends in Plant Science, 30(5), 565–575.

Othman, Y., Bataineh, K., Al-Ajlouni, M., Alsmairat, N., Ayad, J., Shiyab, S., & St. Hilaire, R. (2019). Soilless culture: Management of growing substrate, water, nutrient, salinity, microorganism, and product quality. Fresenius Environmental Bulletin, 28(4), 3249–3260.

Sayara, T., Basheer-Salimia, R., Hawamde, F., & Sánchez, A. (2020). Recycling of organic wastes through composting: Process performance and compost application in agriculture. Agronomy, 10(11), 1838. https://doi.org/10.3390/agronomy10111838

Shahmansouri, S., Mosaddeghi, M. R., & Shariatmadari, H. (2025). Stability of oxygen diffusivity and aeration indicators in soilless growth media under wetting–drying cycles. Agricultural Water Management, 322, 109953. https://doi.org/10.1016/j.agwat.2025.109953

Sim, J. Y., Choi, Y. H., Kim, B., & Park, S. Y. (2025). Toward sustainable fertilizer use: Fundamental analysis of ashes from bamboo by-products. BioResources, 20(3), 1–18.

Taofik, A., Frasetya, B., Nugraha, R., & Sudrajat, A. (2019). The effects of substrate composition on the growth of Brassica oleracea var. acephala with drip hydroponic system. Journal of Physics: Conference Series, 1402(3), 033031. https://doi.org/10.1088/1742-6596/1402/3/033031

Tariq, M. U., Sarwar, M., Anjum, S., Mukhtar, A., Ashraf, M. I., & Hussain, T. (2026). Role of biodegradable substrates in improving productivity and quality of sweet pepper (Capsicum annuum L.). Research Square, 1–21. https://doi.org/10.21203/rs.3.rs-8827011/v1

Tarkowski, Ł. P., Signorelli, S., Considine, M. J., & Montrichard, F. (2023). Integration of reactive oxygen species and nutrient signalling to shape root system architecture. Plant, Cell & Environment, 46(2), 379–390. https://doi.org/10.1111/pce.14504

Tuxun, A., Xiang, Y., Shao, Y., Son, J. E., Yamada, M., Yamada, S., Tagawa, K., Baiyin, B., & Yang, Q. (2025). Soilless cultivation: Precise nutrient provision and growth environment regulation under different substrates. Plants, 14(14), 2203. https://doi.org/10.3390/plants14142203

Vázquez-Hernández, M. C., Parola-Contreras, I., Montoya-Gómez, L. M., Torres-Pacheco, I., Schwarz, D., & Guevara-González, R. G. (2019). Eustressors: Chemical and physical stress factors used to enhance vegetable production. Scientia Horticulturae, 250, 223–229. https://doi.org/10.1016/j.scienta.2019.02.053

Wei, X., Xie, B., Wan, C., Song, R., Zhong, W., Xin, S., & Song, K. (2024). Enhancing soil health and plant growth through microbial fertilizers: Mechanisms, benefits, and sustainable agricultural practices. Agronomy, 14(3), 609. https://doi.org/10.3390/agronomy14030609

Welch, R. M. (2024). Importance of seed mineral nutrient reserves in crop growth and development. In Z. Rengel (Ed.), Mineral nutrition of crops (pp. 205–226). CRC Press.

Wu, X., Wang, J., Yu, Z., Amanze, C., Shen, L., Wu, X., & Zeng, W. (2022). Impact of bamboo sphere amendment on composting performance and microbial community succession in food waste composting. Journal of Environmental Management, 303, 114144. https://doi.org/10.1016/j.jenvman.2021.114144

Xia, Z., Gong, Y., Yang, Y., Wu, M., Bai, J., Zhang, S., & Lu, H. (2024). Effects of root-zone warming, nitrogen supply, and their interactions on root-shoot growth, nitrogen uptake, and photosynthetic physiological characteristics of maize. Plant Physiology and Biochemistry, 214, 108887. https://doi.org/10.1016/j.plaphy.2024.108887

Xu, J., Long, Z., Sun, B., Zhang, F., Shen, J., & Jin, K. (2025). Optimizing root phenotypes for compacted soils: Enhancing root-soil-microbe interactions. Plant, Cell & Environment, 48(6), 4656–4667. https://doi.org/10.1111/pce.15462

Zhang, W., Fang, D., Dong, K., Hu, F., Ye, Z., & Cao, J. (2023). Insights into the environmental factors shaping lateral root development. Physiologia Plantarum, 175(2), e13878. https://doi.org/10.1111/ppl.13878

Published

2026-07-28

How to Cite

Ariansah, P., Fathoni, M. A. N., Krisantini, & Santosa, E. (2026). Substrate porosity and morphological performance identify an effective growing medium for habanero pepper (Capsicum chinense Jacq. cv. Francisca) seedlings. Journal of Agrosociology and Sustainability, 4(1). https://doi.org/10.61511/jassu.v4i1.2026.3893

Issue

Section

Articles

Citation Check