Application of ecoenzymes for the growth of pak choi (Brassica rapa L.) growth in a wick hydroponic system toward sustainable agriculture

Authors

  • Fikrah Fadhilah Janitra Department Biology, Faculty of Mathematics and Natural Sciences Education, Universitas Pendidikan Indonesia, Bandung, West Java 40154, Indonesia
  • Hertien Koosbandiah Surtikanti Department Biology, Faculty of Mathematics and Natural Sciences Education, Universitas Pendidikan Indonesia, Bandung, West Java 40154, Indonesia
  • Kusdianti Department Biology, Faculty of Mathematics and Natural Sciences Education, Universitas Pendidikan Indonesia, Bandung, West Java 40154, Indonesia
  • Afri Irawan Department Biology, Faculty of Mathematics and Natural Sciences Education, Universitas Pendidikan Indonesia, Bandung, West Java 40154, Indonesia

DOI:

https://doi.org/10.61511/hjtas.v4i1.2026.3839

Keywords:

ecoenzymes, pak choi, wick-system hydroponics

Abstract

Background: Pak choi (Brassica rapa L.) is an important leafy vegetable in Indonesia, and sustainable nutrient management remains essential for improving its production. The use of eco-enzymes as an environmentally friendly alternative to liquid organic fertilizers for pak choi nutrition shows promising potential, whether grown in soil or hydroponically, to support sustainable agriculture. This study aims to identify the optimal ecoenzyme formulation as an alternative nutrient source for pak choi (Brassica rapa L.) grown in a wick-type hydroponic system (without water flow). Methods: This study used six treatments, including E0 (hydroponics without nutrients/using water only as the negative control), E1 (1% ecoenzyme), E2 (1.5% ecoenzyme), E3 (2% ecoenzyme), E4 (2.5% ecoenzyme), and E5 (0.5% AB mix). Observations were conducted over eight weeks, with water and nutrient changes performed once a week. The vegetative parameters measured included the number of leaves, plant height, leaf area, leaf color and shape, fresh weight, and dry weight. Findings: The results showed that a 2.5% concentration was the most optimal among the other ecoenzyme treatments for vegetative parameters, including the number of leaves, plant height and the percentage of yellowing leaves. The results indicate an upward trend with increasing concentration until the pH tolerance threshold was reached, revealing the potential of ecoenzymes as an alternative nutrient for pak choi in wick-system hydroponics. Conclusion: This study concluded that a 2.5 % ecoenzyme concentration was suggested for additional nutrition to support pak choi growth in hydroponic wick system, since the result shown still differ significantly from ab mix treatment. Novelty/Originality of this article: This study evaluated ecoenzyme as a standalone nutrient source rather than a fertilizer additive and identified its optimal concentration for supporting vegetative growth of pak choi in a wick hydroponic system.

References

Ali, A., Jabeen, N., Farruhbek, R., Chachar, Z., Laghari, A. A., Chachar, S., Ahmed, N., Ahmed, S., & Yang, Z. (2025). Enhancing nitrogen use efficiency in agriculture by integrating agronomic practices and genetic advances. Frontiers in Plant Science, 16, 1543714. https://doi.org/10.3389/fpls.2025.1543714

Alharbi, K., Alnusairi, G. S., Alnusaire, T. S., Alghanem, S., Alsudays, I. M., Alaklabi, A., & Soliman, M. H. (2024). Potassium silica nanostructure improved growth and nutrient uptake of sorghum plants subjected to drought stress. Frontiers in Plant Science, 15, 1425834. https://doi.org/10.3389/fpls.2024.1425834

Alneyadi, K. S. S., Almheiri, M. S. B., Tzortzakis, N., Di Gioia, F., & Ahmed, Z. F. R. (2024). Organic-based nutrient solutions for sustainable vegetable production in a zero-runoff soilless growing system. Journal of Agriculture and Food Research, 15, 101035. https://doi.org/10.1016/j.jafr.2024.101035

Anggreani, M., Ratih, A., Husaini, M., Emalia, Z., Usman, M., Aida, N., & Ciptawaty, U. (2023). Analisis pengaruh sektor pertaian terhadap PDRB sektor pertanian di Indonesia tahun 2015-2021. Journal on Education, 6(1). https://doi.org/10.31004/joe.v6i1.3871.

Arzita, Setiawan, M. H., Mapegau, & Nizori, A. (2023). Variasi media tanam terhadap pertumbuhan pakcoy (Brassica rapa L.) dengan metode hidroponik. Jurnal Media Pertanian, 8(1). https://jagro.unbari.ac.id/index.php/agro/article/download/188/116

Balasundram, S., Shamshiri, R., Sridhara, S., & Rizan, N. (2023). The role of digital agriculture in mitigating climate change and ensuring food security: An overview. Sustainability. https://doi.org/10.3390/su15065325.

Bihari, C., Ahamad, S., Kumar, M., Kumar, A., Kamboj, A., Singh, S., Srivastava, V., & Gautam, P. (2023). Innovative soilless culture techniques for horticultural crops: A comprehensive review. International Journal of Environment and Climate Change, 13. https://doi.org/10.9734/ijecc/2023/v13i103084.

Castillo‐Díaz, F., Belmonte-Ureña, L., López-Serrano, M., & Camacho‐Ferre, F. (2024). Quantifying sustainability in the agri-food system: A comprehensive methodological framework and expert consensus approach. Agricultural and Food Economics, 12, 1-22. https://doi.org/10.1186/s40100-024-00314-w.

Chen, X., Shuai, C., & Wu, Y. (2022). Global food stability and its socio‐economic determinants towards sustainable development goal 2 (Zero Hunger). Sustainable Development. https://doi.org/10.1002/sd.2482.

Choi, I., Cho, H., Brandizzi, F., & Rouached, H. (2026). Phosphorus deficiency and root growth: the role of TOR signaling in adaptive responses. Journal of Experimental Botany, 77(5), 1375-1383. https://doi.org/10.1093/jxb/eraf204

Ernanda, M. Y., Indrawati, A., & Mardiana, S. (2022). Respon pertumbuhan dan produksi tanaman pakcoy (Brassica rapa L.) terhadap pemberian pupuk organik kandang ayam dan pupuk organik cair (POC) urin sapi. Jurnal Ilmiah Pertanian (JIPERTA), 4(1), 10-19. https://doi.org/10.31289/jiperta.v4i1.1191

Fadlilla, T., Budiastuti, M. S., & Rosariastuti, M. R. (2023). Potential of fruit and vegetable waste as eco-enzyme fertilizer for plants. Jurnal Penelitian Pendidikan IPA, 9(4), 2191-2200. https://doi.org/10.29303/jppipa.v9i4.3010

Fang, S., Wan, Z., Shen, T., & Liang, G. (2024). Potassium attenuates drought damage by regulating sucrose metabolism and gene expression in sesame leaf. Plant Physiology and Biochemistry, 209, 108547. https://doi.org/10.1016/j.plaphy.2024.108547

Fang, X., Yang, Y., Zhao, Z., Zhou, Y., Liao, Y., Guan, Z., Chen, S., Fang, W., Chen, F., & Zhao, S. (2023). Optimum nitrogen, phosphorous, and potassium fertilizer application increased chrysanthemum growth and quality by reinforcing the soil microbial community and nutrient cycling function. Plants (Basel, Switzerland), 12(23), 4062. https://doi.org/10.3390/plants12234062

Fathidarehnijeh, E., Nadeem, M., Cheema, M., Thomas, R., Krishnapillai, M., & Galagedara, L. (2023). Current perspective on nutrient solution management strategies to improve the nutrient and water use efficiency in hydroponic systems. Canadian Journal of Plant Science, 104(2), 88-102. https://doi.org/10.1139/cjps-2023-0034

Hasanah, H., & Wahid, R. (2025). Identifikasi jenis bakteri asam laktat dan uji kualitas ecoenzyme dari sisa kulit buah pada sampah dapur rumah tangga di kelurahan Tondo. Jurnal romotif reventif, 8(1), 17-24. https://doi.org/10.47650/jpp.v8i1.1544

Hernita, D., Poerwanto, R., Susila, A., & Anwar, S. (2021). Determination of phosphorus status on duku (Lansium domesticum) seedling. IOP Conference Series: Earth and Environmental Science, 807. https://doi.org/10.1088/1755-1315/807/3/032027.

Ishfaq M, Wang Y, Yan M, Wang Z, Wu L, Li C and Li X (2022) Physiological essence of magnesium in plants and its widespread deficiency in the farming system of China. Frontiers Plant Science. 13. https://doi.org/10.3389/fpls.2022.802274

Kataria, N., & Singh, N. (2022). Managing water stress by potassium fertilizer in legumes for sustainable agricultural intensification: A review. Legume Research: An International Journal, 45(7). https://doi.org/10.18805/LR-4094

Ladikou, E., Daras, G., Landi, M., Chatzistathis, T., Sotiropoulos, T., Rigas, S., & Papadakis, I. E. (2025). Physiological and biochemical effects of potassium deficiency on apple tree growth. Horticulturae. https://doi.org/10.3390/horticulturae11010042

Liu, Y., Lu, L., Zhang, Y., Yin, Q., Yi, N., Qaseem, M., Li, H., & Wu, A. (2021). Potassium deficiency inhibits leaf-growth and promotes leaf necrotic spots in Neolamarckia cadamba. Tree physiology. https://doi.org/10.1093/treephys/tpab172.

Mishra, M., Desul, S., Santos, C., Mishra, S., Kamal, A., Goswami, S., Kalumba, A., Biswal, R., Da Silva, R., Santos, C., & Baral, K. (2023). A bibliometric analysis of sustainable development goals (SDGs): a review of progress, challenges, and opportunities. Environment, Development and Sustainability, 143. https://doi.org/10.1007/s10668-023-03225-w.

Narayan, O., Kumar, P., Yadav, B., Dua, M., & Johri, A. (2022). Sulfur nutrition and its role in plant growth and development. Plant Signaling & Behavior, 18. https://doi.org/10.1080/15592324.2022.2030082.

Nurfadilah, F., Surtikanti, H. K., & Nilawati, T. S. (2024). Pertumbuhan tanaman bayam horenzo (Spinacia orelacea L.) dengan pemberian nutrisi menggunakan ekoenzim. Holistic: Journal of Tropical Agriculture Sciences, 1(2). https://doi.org/10.61511/hjtas.v1i2.2024.333

Ortel, C. C., Roberts, T. L., & Rupe, J. C. (2024). A review of the interaction between potassium nutrition and plant disease control. Agrosystems, Geosciences & Environment, 7(2), e20489. https://doi.org/10.1002/agg2.20489

Qi, Z., Ling, F., Jia, D., Cui, J., Zhang, Z., Xu, C., Yu, L., Guan, C., Wang, Y., Zhang, M., & Dou, J. (2023). Effects of low nitrogen on seedling growth, photosynthetic characteristics and antioxidant system of rice varieties with different nitrogen efficiencies. Scientific Reports, 13, 19780. https://doi.org/10.1038/s41598-023-47260-z

Radinka, S., Zuhair, N., Nauli, G., Aulia, N., Mundi, C., & Yeninta, D. (2023). Peran mahasiswa dalam menjaga dan membudidayakan tanaman hidroponik di jurusan PKK. Indonesian Journal of Conservation, 12(1). https://journal.unnes.ac.id/nju/index.php/ijc

Razmjooei, Z., Etemadi, M., Eshghi, S., Ramezanian, A., Mirazimi Abarghuei, F., & Alizargar, J. (2022). Potential role of foliar application of azotobacter on growth, nutritional value and quality of lettuce under different nitrogen levels. Plants, 11(3), 406. https://doi.org/10.3390/plants11030406

Ren, M., Li, Y., Zhu, J., Zhao, K., Wu, Z., & Mao, C. (2023). Phenotypes and molecular mechanisms underlying the root response to phosphate deprivation in plants. International Journal of Molecular Sciences, 24(6), 5107. https://doi.org/10.3390/ijms24065107

Rosalina, D. I. (2022). Penerapan dalam pembangunan pertanian modern di Indonesia yang sehat, ramah lingkungan dan berkelanjutan. Semagri, 3(1). https://semagri.upnjatim.ac.id/index.php/semagri/article/view/15

Salsabila, R. K., & Winarsih, W. (2023). Pengaruh pemberian ekoenzim sebagai pupuk organik cair terhadap pertumbuhan tanaman sawi pakcoy (Brassica rapa L.). LenteraBio: Berkala Ilmiah Biologi, 12(1), 50–59. https://doi.org/10.26740/lenterabio.v12n1.p50-59

Saputra, A., Listiana, I., & Saputri, D. A. (2025). Analisis kandungan klorofil pada benih padi tua (Oryza sativa L.) yang terpapar medan magnet extremely low frequency (ELF) pada Masa Pertumbuhan Vegetatif. Bioscientist: Jurnal Ilmiah Biologi, 13(1), 327–335. https://doi.org/10.33394/bioscientist.v13i1.15057

Silva, P., Reis, I., Nascimento, C., Nascimento, C., & Filho, A. (2021). Characterization of growth and visual symptoms of nitrogen, potassium and magnesium deficiencies in arugula. Emirates Journal of Food and Agriculture. https://doi.org/10.9755/ejfa.2021.v33.i7.2722.

Suharjo, U. K. J., Siburian, W. L., & Marlin, M. (2023). Uji enam racikan nutrisi hidroponik pada tanaman pakchoy (Brassica rapa L.) sebagai pengganti larutan AB-Mix. Proceedings Series on Physical & Formal Sciences, 5, 251-259. https://doi.org/10.30595/pspfs.v5i.730

Sumiati, S., Chofifawati, A., & Al Faroqi, N. A. R. (2024). Nutrient deficiency analysis on maize plant morphology. Jurnal Biologi Tropis, 24(2b), 327-339. https://doi.org/10.29303/jbt.v24i2b.7799

Tiljuir, J. N. D., Gafur, M. A. A., & Rosalina, F. (2023). Pengaruh perbedaan dosis nutrisi AB Mix sistem hidroponik rakit apung terhadap pertumbuhan tanaman selada (Lactuca sativa L.). Agriva Journal (Journal of Agriculture and Sylva), 1(1), 26-33. https://ejournal.um-sorong.ac.id/index.php/agriva/article/view/2220

Wahyuningsih, M., Triani, N., & Tarigan, P. L. (2024). The effect of AB mix nutrient concentration and liquid organic fertilizer on growth and yield of pakcoy (Brassica rapa L.) in hydroponic wick system. International Journal of Multidisciplinary Research and Literature, 3(3), 292-299. https://doi.org/10.53067/ijomral.v3i3.220

Wardhani, V. D., Rosyidah, A., & Lestari, M. W. (2025). Hasil berbagai jenis microgreen akibat aplikasi pupuk organik cair. Agronisma, 12(2), 22-31. https://jim.unisma.ac.id/index.php/AGRNM/article/viewFile/26107/19795

Zhou, Z., Zhang, S., Jiang, N., Xiu, W., Zhao, J., & Yang, D. (2022). Effects of organic fertilizer incorporation practices on crops yield, soil quality, and soil fauna feeding activity in the wheat-maize rotation system. Frontiers in Environmental Science, 1058071. https://doi.org/10.3389/fenvs.2022.1058071

Downloads

Published

2026-07-30

How to Cite

Janitra, F. F., Surtikanti, H. K., Kusdianti, & Irawan, A. (2026). Application of ecoenzymes for the growth of pak choi (Brassica rapa L.) growth in a wick hydroponic system toward sustainable agriculture. Holistic: Journal of Tropical Agriculture Sciences, 4(1), 40–58. https://doi.org/10.61511/hjtas.v4i1.2026.3839

Issue

Section

Articles

Citation Check