Bitter leaf extract modulates antioxidant enzymes, immunoglobulins, organ weights, meat oxidation, and hepatic stress markers in broiler chickens exposed to aflatoxin B1
DOI:
https://doi.org/10.61511/hjtas.v3i2.2026.2419Keywords:
hepatoxicity, mycotoxin, oxidative stress, phytochemicals, poultry productsAbstract
Background: This study examined the effects of bitter leaf extract on the oxidative enzymes, stress markers, immunoglobulins, organ weights, meat oxidation, and liver health in broiler chickens exposed to aflatoxin-B1. Methods: 240 day-old mixed-sex Cobb-500 broiler chickens were randomly assigned to the following treatment groups: CONT (Control); BE0AF (0.5 mg/kg aflatoxin-B1); BE1AF (0.5 mg/kg aflatoxin B1 + 1 g bitter leaf powder/ L H2O) or BE2AF (0.5mg/kg aflatoxin-B1 + 2 g bitter leaf powder/ L H2O), each with 60 chickens (6 replicates of 10 chickens). Findings: BE0AF significantly (P<0.05) lowered the serum superoxide dismutase (SOD) and higher malondialdehyde (MDA) levels compared to untreated chickens. BE2AF resulted in similar MDA levels compared to chickens in the BE1AF and CONT groups. BE0AF group showed higher (P<0.05) lactate dehydrogenase (LDH) and lower catalase and glutathione peroxidase (GPx) levels than other groups. Immunoglobulin G levels were notably (P<0.05) lower in the BE0AF group, while immunoglobulins M and A were significantly (P<0.05) lower compared to the BE3AF and BE2AF groups. Liver and spleen weights were higher in the aflatoxin group, and meat catalase was lower (P<0.05) in BE0AF. Lipid and protein oxidation were higher (P<0.05) in BE0AF compared to CONT. Furthermore, liver HSP70, NF-κB, and LDH levels were higher (P<0.05) in BE0AF. Conclusion: Oral administration of bitter leaf extract of 1-2g/ L H2O bitter leaf powder mitigated oxidative stress and protected against aflatoxin-induced damage by improving various physiological markers in broiler chickens. Novelty/Originality of this article: This study was to assess the impact of bitter leaf aqueous extract on organ weights, immunoglobulin levels, serum biomarkers, meat oxidation, hepatic oxidative enzymes, and nuclear factor kappa B in broiler chickens exposed to AFB1.
References
Amminikutty, N., Spalenza, V., Jarriyawattanachaikul, W., Badino, P., Capucchio, M. T., Colombino, E., Schiavone, A., Greco, D., Ascanio, V. D., Avantaggiato, G., Dabbou, S., Nebbia, C., & Girolami, F. (2023). Turmeric Powder Counteracts Oxidative Stress and Reduces AFB1 Content in the Liver of Broilers Exposed to the EU Maximum Levels of the Mycotoxin. Toxins, 1–17. https://doi.org/10.3390/toxins15120687
Chen, Z., Xing, T., Li, J., Zhang, L., Jiang, Y., & Gao, F. (2022). Oxidative stress impairs the meat quality of broiler by damaging mitochondrial function , affecting calcium metabolism and leading to ferroptosis. Animal Bioscience, 35(10), 1616–1627. https://doi.org/10.5713/ab.22.0019.
D’Souza, D., Babu, S., Shetty, S. R., & Balan, P. (2012). Estimation of serum malondialdehyde in potentially malignant disorders and post ‑ antioxidant treated patients : A biochemical study. Contemporary Clinical Dentistry, 3(4), 448–451. https://doi.org/10.4103/0976-237X.107438
Edo, I. G., Oghenekeno, P., Ngukuran, A., Ogheneoruese, F., Ishioma, L., Obasohan, P., Rapheal, A., Electric, J., Ovie, V., Favour, C., Ugbuwe, E., Ongulu, J., Ijide, M., Destiny, I., Ruth, S., & Ewomazino, J. (2023). Biological and bioactive components of bitter leaf (Vernonia amygdalina leaf ): Insight on health and nutritional benefits: A review. Food Chemistry Advances, 3(October), 100488. https://doi.org/10.1016/j.focha.2023.100488
Farombi, E. O., & Owoeye, O. (2011). Antioxidative and Chemopreventive Properties of Vernonia amygdalina and Garcinia biflavonoid. International Journal Environment Research and Public Health, 2533–2555. https://doi.org/10.3390/ijerph8062533
Forni, C., Facchiano, F., Bartoli, M., Pieretti, S., Facchiano, A., Arcangelo, D. D., Norelli, S., Valle, G., Nisini, R., Beninati, S., Tabolacci, C., & Jadeja, R. N. (2019). Beneficial Role of Phytochemicals on Oxidative Stress and Age-Related Diseases. BioMed Research International, 2019(Figure 1). https://doi.org/10.1155/2019/8748253
Fouad, A. M., Ruan, D., El-senousey, H. K., Chen, W., Jiang, S., & Zheng, C. (2019). Harmful Effects and Control Strategies of Aflatoxin B 1 Produced by Aspergillus flavus and Aspergillus parasiticus Strains on Poultry: Review. Toxins, 1, 1–21. https://doi.org/10.3390/toxins11030176
Fratta, P. A. M., & Cominacini, L. (2023). Potential Benefits of Antioxidant Phytochemicals on Endogenous Antioxidants Defences in Chronic Diseases. Antioxidants, 1, 10–15. https://doi.org/10.3390/antiox12040890
Gao, T., Wang, F., Li, S., Luo, X., & Zhang, K. (2011). Manganese regulates manganese-containing superoxide dismutase (MnSOD) expression in the primary broiler myocardial cells. Biological Trace Element Research, 144, 1–3. https://doi.org/10.1007/s12011-011-9093-y
Holovska, K., Boldizarova, K., Cekonova, S., Lenartova, V., Levkut, M., Javorsky, P., & Leng, L. (2003). Antioxidant enzyme activities in liver tissue of chickens fed diets supplemented with various forms and amounts of selenium. Journal of Animal and Feed Sciences, 2, 143–152. https://doi.org/10.22358/jafs/67691/2003
Hua, Z., Liu, R., Chen, Y., Liu, G., Li, C., & Song, Y. (2021). Contamination of A fl atoxins Induces Severe Hepatotoxicity Through Multiple Mechanisms. Front, 11(January), 1–14. https://doi.org/10.3389/fphar.2020.605823
Jobe, M. C., Mthiyane, D. M. N., Dludla, P. V, Mazibuko-mbeje, S. E., Onwudiwe, D. C., & Mwanza, M. (2023). Pathological Role of Oxidative Stress in Aflatoxin-Induced Toxicity in Different Experimental Models and Protective Effect of Phytochemicals : A Review. Molecules. https://doi.org/10.3390/molecules28145369
Kócsó, D. J., Ali, O., Kovács, M., Mézes, M., Balogh, K., Kachlek, M. L., Bóta, B., Zeebone, Y. Y., & Szabó, A. (2021). A preliminary study on changes in heat shock protein 70 levels induced by Fusarium mycotoxins in rats : in vivo study. Mycotoxin Research, 141–148. https://doi.org/10.1007/s12550-021-00425-z
Lee, M. T., Lin, W. C., & Lee, T. T. (2019). Potential crosstalk of oxidative stress and immune response in poultry through phytochemicals — A review. Asian-Australas Journal Animal Science, 32(3), 309–319. https://doi.org/10.5713/ajas.18.0538
Li, C., Liu, X., Wu, J., Ji, X., & Xu, Q. (2022). Research progress in toxicological effects and mechanism of aflatoxin B 1 toxin. PeerJ. https://doi.org/10.7717/peerj.13850
Liu, T., Zhang, L., Joo, D., & Sun, S. (2017). NF- κ B signaling in in fl ammation. Signal Transduction and Targeted Therapy, April. https://doi.org/10.1038/sigtrans.2017.23
Machado, I. F., Miranda, R. G., Dorta, D. J., Rolo, A. P., & Palmeira, C. M. (2023). Targeting Oxidative Stress with Polyphenols to Fight Liver Diseases. Antioxidants. https://doi.org/10.3390/antiox12061212
Muhlisin, Utama, T. D., Lee, J. H., Choi, J. H., & Lee, S. K. (2016). Antioxidant Enzyme Activity, Iron Content and Lipid Oxidation of Raw and Cooked Meat of Korean Native Chickens and Other Poultry. Asian Australas. Journal Animal Bioscience., 29(5), 695–701. https://doi.org/10.5713/ajas.15.0256
Olarotimi, O. J., Gbore, F. A., Adu, O. A., Oloruntola, O. D., & Jimoh, O. A. (2023). Ameliorative effects of Sida acuta and vitamin C on serum DNA damage, pro-inflammatory and anti-inflammatory cytokines in roosters fed aflatoxin B₁-contaminated diets. Toxicon. https://doi.org/10.1016/j.toxicon.2023.107330
Oloruntola, O. D. (2024). Juglans kernel powder and jacobinia leaf powder supplementation influenced growth , meat , brain , immune system and DNA biomarker of broiler chickens fed Aflatoxin-B1 contaminated diets. Biotech Studies, 33(1), 33–42. https://doi.org/10.38042/biotechstudies.1442037
Oloruntola, O. D., Ayodele, S. O., Oloruntola, D. A., Olarotimi, O., Falowo, A., Akinduro, V. O., Gbore, F. A., Adu, O. A., & Agbede, J. O. (2024). Dietary supplementation of Capsicum powder affects the growth, immunoglobulins, pro-inflammatory cytokines, adipokines, meat, and liver histology of aflatoxin B₁ exposed broiler chickens. Toxicon, 240. https://doi.org/10.1016/j.toxicon.2024.107640
Payne, R. L., & Southern, L. L. (1997). Changes in Glutathione Peroxidase and Tissue Selenium Concentrations of Broilers After Consuming a Diet Adequate in Selenium 1 , 2. Poultry Science, 84(8), 1268–1276. https://doi.org/10.1093/ps/84.8.1268
Souza, M. A. A., Visentainer, J. V, Carvalho, R. H., Garcia, F., Elza, I., & Shimokomaki, M. (2013). Lipid and Protein Oxidation in Charqui Meat and Jerked Beef. Brazilian Archives of Biology and Technology, 56(February), 107–112. https://doi.org/10.1590/S1516-89132013000100014
Surai, P. F., Kochish, I. I., Fisinin, V. I., & Kidd, M. T. (2019). Antioxidant Defence Systems and Oxidative Stress in Poultry Biology : An Update. Antioxidants, 1–36. https://doi.org/10.3390/antiox8070235
Tokofai, B. M., Idoh, K., & Oke, O. E. (2021). Extract on CCl 4 -Induced Liver Injury in Broiler Chickens. Animalsanimals, 1–11. https://doi.org/10.3390/ani11123371
Tokur, B., Korkmaz, K., & Ayas, D. (2006). Comparison of Two Thiobarbituric Acid ( TBA ) Method for Monitoring Lipid Oxidation in Fish. E.U. Journal of Fisheries & Aquatic Sciences, 23(1960), 331–334. http://www.egejfas.org/en/pub/article/67903
Ugbogu, E. A., Emmanuel, O., Dike, E. D., Agi, G. O., Ugbogu, O. C., Ibe, C., & Iweala, E. J. (2021). The Phytochemistry , Ethnobotanical , and Pharmacological Potentials of the Medicinal Plant- Vernonia amygdalina L . ( bitter Leaf ). Clinical Complementary Medicine and Pharmacology, 1(1), 100006. https://doi.org/10.1016/j.ccmp.2021.100006
Vipin, Rao, R., Kurrey, N. K., Appaiah, A., & Venkateswaran. (2017). Protective effects of phenolic-rich extract of ginger against aflatoxin B₁-induced oxidative stress and hepatotoxicity. Biomedicine & Pharmacotherapy. Biomed Pharmacother. https://doi.org/10.1016/j.biopha.2017.04.107
Zhong, R., Miao, L., Zhang, H., Tan, L., Zhao, Y., Tu, Y., Angel, M., Simal-gandara, J., Chen, L., & He, C. (2022). Anti-inflammatory activity of flavonols via inhibiting MAPK and NF- κ B signaling pathways in RAW264 . 7 macrophages. Current Research in Food Science, 5(March), 1176–1184. https://doi.org/10.1016/j.crfs.2022.07.007
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